Hotspot

trnF-GAA-ndhJ

LSC · 55125-56245 · 1121 bp

Back to Arisaema

Pi 0.0556

MarkerSeek score 90.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 1121
variable_sites: 195
pi: 0.0556
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	883-1618 bp	1.0	90.6
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	926-1661 bp	1.0	89.3
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	925-1660 bp	1.0	89.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	914-1649 bp	1.0	88.4
trnF-GAA-ndhJ_p5	TGGAAAATGGTCGGGATAGC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.7	57.9	931-1666 bp	1.0	88.2