Hotspot

trnT-UGU-trnL-UAA

LSC · 52438-53956 · 1519 bp

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Pi 0.0849

MarkerSeek score 84.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1519
variable_sites: 388
pi: 0.0849
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TCCATACCAAGGCTCAATGC	55.0	50.0	57.9	57.9	1058-2436 bp	1.0	90.5
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ACCAAGGCTCAATGCAATCA	55.0	45.0	57.9	58.1	1053-2431 bp	1.0	90.4
trnT-UGU-trnL-UAA_p3	GAGTCGAACCGATGACCATC	TCCATACCAAGGCTCAATGC	55.0	50.0	58.2	57.9	1089-2467 bp	1.0	90.3
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	ACCAAGGCTCAATGCAATCA	55.0	45.0	58.2	58.1	1084-2462 bp	1.0	90.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	1025-2403 bp	1.0	89.7