Hotspot

rps4-trnT-UGU

LSC · 51167-52364 · 1198 bp

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Pi 0.0893

MarkerSeek score 74.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 289
pi: 0.0893
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCAAATGTTTCGGCACCT	GCACGAGCTTGAGATGAGAA	45.0	50.0	58.0	58.0	1142-1876 bp	0.923077	88.8
rps4-trnT-UGU_p2	ATCCGACCCTTCCCTTGTTA	GCACGAGCTTGAGATGAGAA	50.0	50.0	58.0	58.0	1284-2018 bp	0.923077	88.6
rps4-trnT-UGU_p3	TCCGACCCTTCCCTTGTTAT	GCACGAGCTTGAGATGAGAA	50.0	50.0	58.0	58.0	1283-2017 bp	0.923077	88.6
rps4-trnT-UGU_p4	TGTGATCTTTTACCGGAGCG	GCACGAGCTTGAGATGAGAA	50.0	50.0	58.0	58.0	775-1509 bp	0.846154	85.8
rps4-trnT-UGU_p5	ATCTTTTACCGGAGCGCAAT	GCACGAGCTTGAGATGAGAA	45.0	50.0	58.0	58.0	771-1505 bp	0.846154	85.5