Hotspot

psbK-psbI

LSC · 8948-9427 · 480 bp

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Pi 0.0213

MarkerSeek score 75.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 45
pi: 0.0213
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGGTTACGTCCTGGATCAT	50.0	50.0	57.9	57.8	589-621 bp	1.0	87.2
psbK-psbI_p2	GTGGACATTATGCCCGTCAT	AGGGTTACGTCCTGGATCAT	50.0	50.0	58.0	57.8	637-669 bp	1.0	86.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	AGGGTTACGTCCTGGATCAT	45.0	50.0	58.3	57.8	586-618 bp	1.0	85.3
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	AGGGTTACGTCCTGGATCAT	45.0	50.0	58.3	57.8	587-619 bp	1.0	85.3
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	AGGGTTACGTCCTGGATCAT	45.0	50.0	58.3	57.8	588-620 bp	1.0	85.2