Hotspot

rpoC1

LSC · 21879-24671 · 2793 bp

Pi 0.0012

MarkerSeek score 57.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2793
variable_sites: 6
pi: 0.0012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GGTGAACTGGAGAAACCCAA	TTTGGCTCTGGAACTGAACC	50.0	50.0	57.6	58.0	2953-2959 bp	1.0	77.3
rpoC1_p2	GAAACCCAAAATGACACGGG	TTTGGCTCTGGAACTGAACC	50.0	50.0	57.6	58.0	2942-2948 bp	1.0	76.7
rpoC1_p3	GGTGAACTGGAGAAACCCAA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	2959-2965 bp	1.0	76.7
rpoC1_p4	AGGTGAACTGGAGAAACCCA	TTTGGCTCTGGAACTGAACC	50.0	50.0	58.5	58.0	2954-2960 bp	1.0	76.3
rpoC1_p5	GAAACCCAAAATGACACGGG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	2948-2954 bp	1.0	76.1