Hotspot

rpl14-rpl16

LSC · 84979-85123 · 145 bp

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Pi 0.00345
MarkerSeek score 56.20
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
145
variable_sites
1
pi
0.00345
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 CCGCTGTTATCCGCTACATT TGAAATGAGCGGAGTAGCAG 50.0 50.0 58.1 57.7 285-286 bp 1.0 77.4
rpl14-rpl16_p2 TACACATCAATTCTCGGGCC TGAAATGAGCGGAGTAGCAG 50.0 50.0 57.7 57.7 305-306 bp 1.0 75.8
rpl14-rpl16_p3 CCGCTGTTATCCGCTACATT GAAATGAGCGGAGTAGCAGA 50.0 50.0 58.1 57.4 284-285 bp 1.0 75.1
rpl14-rpl16_p4 TACACATCAATTCTCGGGCC GAAATGAGCGGAGTAGCAGA 50.0 50.0 57.7 57.4 304-305 bp 1.0 73.5
rpl14-rpl16_p5 TCCGCTACATTCAAATGGGT TGAAATGAGCGGAGTAGCAG 45.0 50.0 57.2 57.7 276-277 bp 1.0 72.1