Hotspot

psbZ-trnG-GCC

LSC · 38941-39363 · 423 bp

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Pi 0.00552
MarkerSeek score 53.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
423
variable_sites
4
pi
0.00552
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.7 57.8 581-611 bp 1.0 79.0
psbZ-trnG-GCC_p2 GGGTTGGTCGGGTAACAAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 567-597 bp 1.0 78.8
psbZ-trnG-GCC_p3 TTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 537-567 bp 1.0 73.3
psbZ-trnG-GCC_p4 AGGGTTGGTCGGGTAACAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 59.2 57.8 568-598 bp 1.0 72.0
psbZ-trnG-GCC_p5 ATTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 47.6 50.0 58.2 57.8 582-612 bp 1.0 71.6