Hotspot

rpoB-trnC-GCA

LSC · 27910-29122 · 1213 bp

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Pi 0.0126
MarkerSeek score 60.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1213
variable_sites
30
pi
0.0126
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TAAGCCCTGCTCAATGAACC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1368-1374 bp 1.0 81.2
rpoB-trnC-GCA_p2 TAAGCCCTGCTCAATGAACC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1350-1356 bp 1.0 79.0
rpoB-trnC-GCA_p3 TATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1302-1308 bp 1.0 78.7
rpoB-trnC-GCA_p4 CTCTGTTAAGCCCTGCTCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1374-1380 bp 1.0 78.4
rpoB-trnC-GCA_p5 GTTCCTCTGTTAAGCCCTGC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.5 58.1 1378-1384 bp 1.0 77.8