Hotspot

rpl32-trnL-UAG

SSC · 114529-115241 · 713 bp

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Pi 0.0415
MarkerSeek score 84.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
713
variable_sites
130
pi
0.0415
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 792-974 bp 1.0 90.5
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 793-975 bp 1.0 90.5
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 802-984 bp 1.0 89.6
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 801-983 bp 1.0 89.0
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 800-982 bp 1.0 89.0