Hotspot

ndhF-rpl32

SSC · 113227-114369 · 1143 bp

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Pi 0.0571
MarkerSeek score 85.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1143
variable_sites
215
pi
0.0571
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.95
markerseek_score
85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAACGGT TGAACTAGAGGTGCGCTTTT 50.0 45.0 57.8 57.5 967-1231 bp 1.0 86.2
ndhF-rpl32_p2 CTGGAAGTGGAATGAACGGT TTGAACTAGAGGTGCGCTTT 50.0 45.0 57.8 57.5 968-1232 bp 1.0 86.2
ndhF-rpl32_p3 CTGGAAGTGGAATGAACGGT TTTGAACTAGAGGTGCGCTT 50.0 45.0 57.8 57.5 969-1233 bp 1.0 86.2
ndhF-rpl32_p4 CTGGAAGTGGAATGAACGGT TTTTGAACTAGAGGTGCGCT 50.0 45.0 57.8 57.5 970-1234 bp 1.0 86.2
ndhF-rpl32_p5 CTGGAAGTGGAATGAACGGT TGAACTAGAGGTGCGCTTTTT 50.0 42.9 57.8 58.2 967-1231 bp 1.0 81.3