Hotspot

rpl14-rpl16

LSC · 81836-81971 · 136 bp

Back to Argentina

Pi 0.0523

MarkerSeek score 84.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 22
pi: 0.0523
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	CAATAGCGGCATCAAAAATGC	50.0	42.9	58.1	57.2	230-242 bp	1.0	76.0
rpl14-rpl16_p2	TACACATCAATTCTCGGGCC	CAATAGCGGCATCAAAAATGC	50.0	42.9	57.7	57.2	250-262 bp	1.0	73.8
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	AATAGCGGCATCAAAAATGC	50.0	40.0	58.1	55.7	229-241 bp	1.0	72.1
rpl14-rpl16_p4	TCCGCTACATTCAAATGGGT	CAATAGCGGCATCAAAAATGC	45.0	42.9	57.2	57.2	221-233 bp	1.0	70.8
rpl14-rpl16_p5	TACACATCAATTCTCGGGCC	AATAGCGGCATCAAAAATGC	50.0	40.0	57.7	55.7	249-261 bp	1.0	69.9