Hotspot

rpl36-rps8

LSC · 80381-80877 · 497 bp

Back to Argentina

Pi 0.0386

MarkerSeek score 80.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 80
pi: 0.0386
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CGAACGGAGGCCCTTATTTT	GAATCGGCGGGGAAATTTTG	50.0	50.0	58.3	58.1	467-588 bp	1.0	89.3
rpl36-rps8_p2	TTACTATAATTCGCCCCCGC	GAATCGGCGGGGAAATTTTG	50.0	50.0	57.5	58.1	520-641 bp	1.0	87.7
rpl36-rps8_p3	TACGAACGGAGGCCCTTATT	GAATCGGCGGGGAAATTTTG	50.0	50.0	58.5	58.1	469-590 bp	1.0	87.3
rpl36-rps8_p4	CGAACGGAGGCCCTTATTTT	GAATAGAAGGAATCGGCGGG	50.0	55.0	58.3	58.2	476-597 bp	0.933333	85.7
rpl36-rps8_p5	GCCTACGGATCAATCGACAT	GAATCGGCGGGGAAATTTTG	50.0	50.0	57.6	58.1	502-623 bp	0.933333	85.2