Hotspot

accD-psaI

LSC · 59669-60097 · 429 bp

Back to Argentina

Pi 0.0528
MarkerSeek score 82.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
429
variable_sites
87
pi
0.0528
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCTTTCCTTTGGATCAAACTT CCATTGCAATTGCCGGAAAT 33.3 45.0 53.2 58.0 513-627 bp 0.933333 49.3
accD-psaI_p2 TCTTTCCTTTGGATCAAACTT TTGCCGGAAATACTAAGCCC 33.3 50.0 53.2 58.0 504-618 bp 0.933333 49.3
accD-psaI_p3 TCTTTCCTTTGGATCAAACT CCATTGCAATTGCCGGAAAT 35.0 45.0 52.2 58.0 513-627 bp 0.866667 46.7
accD-psaI_p4 TCTTTCCTTTGGATCAAACT TTGCCGGAAATACTAAGCCC 35.0 50.0 52.2 58.0 504-618 bp 0.866667 46.7
accD-psaI_p5 TTCTTTCCTTTGGATCAAACT CCATTGCAATTGCCGGAAAT 33.3 45.0 53.2 58.0 514-628 bp 0.866667 46.7