Hotspot

trnF-GAA-ndhJ

LSC · 49261-49992 · 732 bp

Back to Argentina

Pi 0.0335

MarkerSeek score 85.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 98
pi: 0.0335
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	857-950 bp	1.0	91.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	821-914 bp	1.0	91.1
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	829-922 bp	1.0	90.9
trnF-GAA-ndhJ_p4	AATGAGGATGGTGTGTCGTG	ATCCACGCCTGAAACGTATC	50.0	50.0	57.9	58.1	884-977 bp	1.0	90.7
trnF-GAA-ndhJ_p5	AGGATGGTGTGTCGTGAATG	ATCCACGCCTGAAACGTATC	50.0	50.0	57.9	58.1	880-973 bp	1.0	90.7