Hotspot

trnG-GCC-trnfM-CAU

LSC · 37089-37298 · 210 bp

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Pi 0.059
MarkerSeek score 80.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
210
variable_sites
51
pi
0.059
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 252-302 bp 1.0 91.2
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 253-303 bp 1.0 91.2
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 250-300 bp 1.0 90.1
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 251-301 bp 1.0 90.1
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.1 57.8 272-322 bp 1.0 89.7