Hotspot

atpH-atpI

LSC · 13531-14468 · 938 bp

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Pi 0.0466

MarkerSeek score 83.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 149
pi: 0.0466
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCATGGAAGGTCATCATTGA	45.0	42.9	58.0	56.7	977-1027 bp	1.0	73.2
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TCCATGGAAGGTCATCATTGA	45.0	42.9	58.2	56.7	978-1028 bp	1.0	72.1
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TCCATGGAAGGTCATCATTGA	50.0	42.9	57.8	56.7	992-1042 bp	1.0	71.8
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TCCATGGAAGGTCATCATTGA	50.0	42.9	58.3	56.7	987-1037 bp	1.0	71.4
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TCCATGGAAGGTCATCATTGA	45.0	42.9	57.6	56.7	988-1038 bp	1.0	70.2