Hotspot

trnS-GGA-trnG-UCC

LSC · 8513-9066 · 554 bp

Back to Argentina

Pi 0.0382

MarkerSeek score 81.00

Primer available yes

Label trnS-GGA-trnG-UCC

Feature Metadata

label_name: trnS-GGA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 80
pi: 0.0382
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	592-636 bp	1.0	63.7
trnS-GGA-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	591-635 bp	1.0	63.7
trnS-GGA-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	595-639 bp	1.0	62.6
trnS-GGA-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	594-638 bp	1.0	62.6
trnS-GGA-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	593-637 bp	1.0	62.6