Hotspot

trnQ-UUG-psbK

LSC · 7334-7747 · 414 bp

Back to Argentina

Pi 0.0416

MarkerSeek score 81.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 63
pi: 0.0416
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	GGGCAATTTGGCTACGAAAA	50.0	45.0	58.7	57.3	523-581 bp	1.0	80.7
trnQ-UUG-psbK_p2	GAATAGCGGGACCAAAACCC	GGGCAATTTGGCTACGAAAA	55.0	45.0	58.9	57.3	532-590 bp	1.0	78.9
trnQ-UUG-psbK_p3	AAAACCCGTTGCCTTACCAC	GGGCAATTTGGCTACGAAAA	50.0	45.0	59.0	57.3	519-577 bp	1.0	78.4
trnQ-UUG-psbK_p4	ACCAAAACCCGTTGCCTTAC	GGGCAATTTGGCTACGAAAA	50.0	45.0	59.0	57.3	522-580 bp	1.0	78.4
trnQ-UUG-psbK_p5	CAAAACCCGTTGCCTTACCA	GGGCAATTTGGCTACGAAAA	50.0	45.0	59.0	57.3	520-578 bp	1.0	78.4