Hotspot

trnK-UUU-rps16

LSC · 4306-5121 · 816 bp

Back to Argentina

Pi 0.0419
MarkerSeek score 75.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
816
variable_sites
130
pi
0.0419
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 889-1088 bp 1.0 91.7
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 55.0 55.0 58.0 58.0 983-1182 bp 1.0 91.6
trnK-UUU-rps16_p3 CAGCGGAAAAGCTTTTACCG AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 1238-1373 bp 0.466667 68.5
trnK-UUU-rps16_p4 CAGCGGAAAAGCTTTTACCG CGACGAGAGGGAAGAGATCT 50.0 55.0 58.0 58.0 1332-1467 bp 0.466667 68.1
trnK-UUU-rps16_p5 CAACGGGCTCTCTCTCTCTA AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1577-1627 bp 0.133333 46.7