Hotspot

trnH-psbA

LSC · 74-428 · 355 bp

Back to Argentina

Pi 0.105

MarkerSeek score 78.00

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 109
pi: 0.105
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	289-452 bp	1.0	90.4
trnH-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	339-502 bp	0.933333	88.0
trnH-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	353-516 bp	0.933333	88.0
trnH-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	352-515 bp	0.933333	88.0
trnH-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	354-517 bp	0.933333	88.0