Hotspot

rpl16

LSC · 84738-86492 · 1755 bp

Back to Arenga

Pi 0.0207
MarkerSeek score 67.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1755
variable_sites
76
pi
0.0207
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATCCTCAATATCCCTTTTGT AGGTTGAATAAAAATTCGATTGACCA 36.4 30.8 54.8 58.1 1792-1892 bp 1.0 47.3
rpl16_p2 TCATCCTCAATATCCCTTTTGT GGTTGAATAAAAATTCGATTGACCA 36.4 32.0 54.8 56.9 1791-1891 bp 1.0 47.3
rpl16_p3 TCCCTTTTGTTTAGTTCTACATCC AGGTTGAATAAAAATTCGATTGACCA 37.5 30.8 56.6 58.1 1780-1880 bp 1.0 47.3
rpl16_p4 TCCCTTTTGTTTAGTTCTACATCC GGTTGAATAAAAATTCGATTGACCA 37.5 32.0 56.6 56.9 1779-1879 bp 1.0 47.3
rpl16_p5 TCATCCTCAATATCCCTTTTGTT AGGTTGAATAAAAATTCGATTGACCA 34.8 30.8 55.6 58.1 1792-1892 bp 1.0 47.3