Hotspot

psaC-ndhE

SSC · 121558-122416 · 859 bp

Pi 0.0709

MarkerSeek score 92.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 859
variable_sites: 109
pi: 0.0709
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 92.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	808-1078 bp	1.0	86.0
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	807-1077 bp	1.0	86.0
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	828-1098 bp	1.0	85.9
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	806-1076 bp	1.0	84.9
psaC-ndhE_p5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	55.0	50.0	58.3	57.4	783-1053 bp	1.0	84.5