Hotspot

ndhF-rpl32

SSC · 117224-117654 · 431 bp

Back to Arenga

Pi 0.0373
MarkerSeek score 70.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
431
variable_sites
34
pi
0.0373
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CATACGTGGAGGGGGAATTG TCAGCGGGCTATGTCAATTC 55.0 50.0 58.0 58.0 1325-1449 bp 1.0 85.6
ndhF-rpl32_p2 AGCAACTGCACCGACAAATA TCAGCGGGCTATGTCAATTC 45.0 50.0 58.1 58.0 1296-1420 bp 1.0 85.0
ndhF-rpl32_p3 ATTTAGCAACTGCACCGACA TCAGCGGGCTATGTCAATTC 45.0 50.0 58.1 58.0 1300-1424 bp 1.0 85.0
ndhF-rpl32_p4 TAGCAACTGCACCGACAAAT TCAGCGGGCTATGTCAATTC 45.0 50.0 58.1 58.0 1297-1421 bp 1.0 85.0
ndhF-rpl32_p5 CATACGTGGAGGGGGAATTG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 1906-2214 bp 1.0 84.7