Hotspot

rpoB-trnC-GCA

LSC · 27921-29156 · 1236 bp

Back to Arenga

Pi 0.014
MarkerSeek score 64.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1236
variable_sites
36
pi
0.014
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1333-1384 bp 1.0 80.1
rpoB-trnC-GCA_p2 TCCCTCATTTCCATTCCGGA TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.4 57.5 1253-1304 bp 1.0 78.6
rpoB-trnC-GCA_p3 TTCCCTCATTTCCATTCCGG TAAAGGATTTGCAGTCCCCC 50.0 50.0 57.6 57.5 1254-1305 bp 1.0 78.4
rpoB-trnC-GCA_p4 AATTCTTCCGTCAAGCCCTG TTTGTTGATCAGGCGACACC 50.0 50.0 58.2 58.8 1370-1421 bp 1.0 78.1
rpoB-trnC-GCA_p5 AATTCTTCCGTCAAGCCCTG GACACCCGGATTTGAACTGG 50.0 55.0 58.2 58.8 1356-1407 bp 1.0 77.6