Hotspot

trnS-GCU-trnG-UCC

LSC · 8550-10040 · 1491 bp

Back to Arenga

Pi 0.0451
MarkerSeek score 74.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1491
variable_sites
122
pi
0.0451
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.85
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA TACCACTAAACTATACCCGC 45.0 45.0 58.0 54.0 749-1551 bp 1.0 59.1
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA TTACCACTAAACTATACCCGC 45.0 42.9 58.0 54.9 750-1552 bp 1.0 58.0
trnS-GCU-trnG-UCC_p3 AGAAGAAGCGGAGAGAGAGG TACCACTAAACTATACCCGC 55.0 45.0 58.2 54.0 805-1607 bp 1.0 57.2
trnS-GCU-trnG-UCC_p4 CGGAGAGAGAGGGATTCGAA TACCACTAAACTATACCCGC 55.0 45.0 58.3 54.0 797-1599 bp 1.0 56.6
trnS-GCU-trnG-UCC_p5 AGAAGAAGCGGAGAGAGAGG TTACCACTAAACTATACCCGC 55.0 42.9 58.2 54.9 806-1608 bp 1.0 56.1