Hotspot

matK-trnK-UUU

LSC · 3243-4175 · 933 bp

Back to Arenga

Pi 0.0282
MarkerSeek score 69.90
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
933
variable_sites
55
pi
0.0282
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCTAAATATCCTTGTAATTCTTCCA ACGGTAGAGTACTCGGCTTT 28.0 50.0 53.8 58.2 886-1031 bp 1.0 48.7
matK-trnK-UUU_p2 TCTAAATATCCTTGTAATTCTTCCA AACGGTAGAGTACTCGGCTT 28.0 50.0 53.8 58.2 887-1032 bp 1.0 48.6
matK-trnK-UUU_p3 TTCTAAATATCCTTGTAATTCTTCCA AACGGTAGAGTACTCGGCTT 26.9 50.0 54.5 58.2 888-1033 bp 1.0 48.6
matK-trnK-UUU_p4 TTCTAAATATCCTTGTAATTCTTCCA ACGGTAGAGTACTCGGCTTT 26.9 50.0 54.5 58.2 887-1032 bp 1.0 48.6
matK-trnK-UUU_p5 TCTAAATATCCTTGTAATTCTTCCA GGGTTGCTAACTCAACGGTA 28.0 50.0 53.8 57.5 900-1045 bp 1.0 48.6