Hotspot

ycf1

SSC · 117922-123522 · 5601 bp

Back to Arenaria

Pi 0.0799

MarkerSeek score 77.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 670
pi: 0.0799
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCACTCAGCACGTTGGAATA	GGTTCCATGCGTGTTCAAAG	50.0	50.0	57.9	57.9	420 bp	0.666667	65.7
ycf1_p2	ATAATCGGGTCTTGGCATCG	GGTTCCATGCGTGTTCAAAG	50.0	50.0	57.8	57.9	594 bp	0.666667	65.0
ycf1_p3	GCATCGATCTATGCCAAGGT	GGTTCCATGCGTGTTCAAAG	50.0	50.0	57.8	57.9	580 bp	0.666667	65.0
ycf1_p4	AAGAAGGTTGTTTTCGGGGG	GGTTCCATGCGTGTTCAAAG	50.0	50.0	58.3	57.9	706 bp	0.666667	64.0
ycf1_p5	CTTGGGTTGGGTTTACCGTT	GGTTCCATGCGTGTTCAAAG	50.0	50.0	58.3	57.9	910 bp	0.666667	63.9