Hotspot

rpoA

LSC · 73327-74346 · 1020 bp

Back to Arenaria

Pi 0.0699

MarkerSeek score 84.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 107
pi: 0.0699
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCAGCATGTACGGCTCTTTC	TACGCGCTATTCGTAGAAGC	50.0	50.0	58.0	58.0	1844-1845 bp	0.666667	66.5
rpoA_p2	GCTCTTTCGGGGGATGAATT	TACGCGCTATTCGTAGAAGC	50.0	50.0	57.9	58.0	1832-1833 bp	0.666667	66.1
rpoA_p3	CTTTTGCAACGCCTTTGGAA	TACGCGCTATTCGTAGAAGC	45.0	50.0	58.1	58.0	1588-1589 bp	0.666667	66.1
rpoA_p4	TCAGCATGTACGGCTCTTTC	GCCCCATAATGGCTGTAGAC	50.0	55.0	58.0	58.1	1786-1787 bp	0.666667	65.9
rpoA_p5	CCGCATGGCCTAATGATCTT	TACGCGCTATTCGTAGAAGC	50.0	50.0	58.1	58.0	1702-1703 bp	0.666667	65.8