Hotspot

rpl16

LSC · 76496-77714 · 1219 bp

Back to Arenaria

Pi 0.0763

MarkerSeek score 84.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1219
variable_sites: 139
pi: 0.0763
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTCATGTCCGCGTTTCGTA	ATGGATAAGAGAGGGACGGG	45.0	55.0	58.0	58.0	2145-2161 bp	0.666667	66.7
rpl16_p2	TATTCATGTCCGCGTTTCGT	ATGGATAAGAGAGGGACGGG	45.0	55.0	58.0	58.0	2146-2162 bp	0.666667	66.7
rpl16_p3	TTCATGTCCGCGTTTCGTAT	ATGGATAAGAGAGGGACGGG	45.0	55.0	58.0	58.0	2144-2160 bp	0.666667	66.7
rpl16_p4	CGCACCAAAAACTCGAGTTC	ATGGATAAGAGAGGGACGGG	50.0	55.0	58.0	58.0	1900-1916 bp	0.666667	66.6
rpl16_p5	TTCATGTCCGCGTTTCGTAT	CAGGCCGTATCGATGGAAAA	45.0	50.0	58.0	58.1	2181-2197 bp	0.666667	66.4