Hotspot

rpl32-trnL-UAG

SSC · 107537-108324 · 788 bp

Back to Arenaria

Pi 0.127

MarkerSeek score 75.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 90
pi: 0.127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTATTGGGCGGCGTTAAAAG	GAGCTGCATAACCCAGTACC	50.0	55.0	57.8	58.0	1198-1539 bp	0.666667	68.9
rpl32-trnL-UAG_p2	GTATTGGGCGGCGTTAAAAG	AGGCCTGAAGTAGCAAAACC	50.0	50.0	57.8	58.1	1100-1441 bp	0.666667	68.6
rpl32-trnL-UAG_p3	GTATTGGGCGGCGTTAAAAG	GGCCTGAAGTAGCAAAACCT	50.0	50.0	57.8	58.1	1099-1440 bp	0.666667	68.6
rpl32-trnL-UAG_p4	GTATTGGGCGGCGTTAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	589-930 bp	0.666667	68.3
rpl32-trnL-UAG_p5	GTATTGGGCGGCGTTAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	582-923 bp	0.666667	67.8