Hotspot

atpB-rbcL

LSC · 51085-51825 · 741 bp

Back to Arenaria

Pi 0.0787

MarkerSeek score 84.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 87
pi: 0.0787
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTAATTGGTTGGCCAGCAGT	CGGTCTCTCCAACGCATAAA	45.0	50.0	58.0	58.0	1570 bp	0.666667	66.7
atpB-rbcL_p2	TTAATTGGTTGGCCAGCAGT	TTGAGGCGTTACTCGGAATG	45.0	50.0	58.0	58.0	1055 bp	0.666667	66.7
atpB-rbcL_p3	TTAATTGGTTGGCCAGCAGT	GTCGGTCCATACAGTTGTCC	45.0	55.0	58.0	58.0	1136 bp	0.666667	66.7
atpB-rbcL_p4	TTAATTGGTTGGCCAGCAGT	GTTGAGGCGTTACTCGGAAT	45.0	50.0	58.0	58.0	1056 bp	0.666667	66.7
atpB-rbcL_p5	GGAGGAAAAGCTACGTCCAG	TTGAGGCGTTACTCGGAATG	55.0	50.0	58.0	58.0	986 bp	0.666667	66.7