Hotspot

trnT-UGU-trnL-UAA

LSC · 43740-44343 · 604 bp

Back to Arenaria

Pi 0.131

MarkerSeek score 82.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 118
pi: 0.131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AACTAATTGGCGAGCTCCAG	AGATCTATCAGACGGTGGGG	50.0	55.0	58.0	58.0	1723-1736 bp	0.666667	66.6
trnT-UGU-trnL-UAA_p2	AAAACCTGACCTGTTGACCC	AGATCTATCAGACGGTGGGG	50.0	55.0	57.9	58.0	1638-1651 bp	0.666667	66.4
trnT-UGU-trnL-UAA_p3	TTTTCTGGCGATACGAACGT	AGATCTATCAGACGGTGGGG	45.0	55.0	57.9	58.0	1612-1625 bp	0.666667	66.3
trnT-UGU-trnL-UAA_p4	ATCTTGGGGTTTGCAACGAT	AGATCTATCAGACGGTGGGG	45.0	55.0	58.1	58.0	1792-1805 bp	0.666667	66.3
trnT-UGU-trnL-UAA_p5	AACTAATTGGCGAGCTCCAG	AGCCAATTAAGTCCGTAGCG	50.0	50.0	58.0	58.1	1276-1289 bp	0.666667	66.2