Hotspot

psbZ-trnG-GCC

LSC · 33456-33756 · 301 bp

Back to Arenaria

Pi 0.159
MarkerSeek score 81.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
301
variable_sites
72
pi
0.159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGGATTAGTTTTTCTGGTGGGT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 57.5 57.8 377-395 bp 1.0 70.9
psbZ-trnG-GCC_p2 AGTTTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 56.5 57.8 371-389 bp 1.0 70.9
psbZ-trnG-GCC_p3 AGTTTTTCTGGTGGGTATCCTT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 57.3 57.8 371-389 bp 1.0 68.7
psbZ-trnG-GCC_p4 AGTTTTTCTGGTGGGTATCC CGTCTTCTCCTTGGCAAAGA 45.0 50.0 54.9 57.8 371-389 bp 1.0 66.0
psbZ-trnG-GCC_p5 GTTTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 54.9 57.8 370-388 bp 1.0 66.0