Hotspot

psbM-trnD-GUC

LSC · 27575-28074 · 500 bp

Back to Arenaria

Pi 0.112
MarkerSeek score 82.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
500
variable_sites
83
pi
0.112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGCGCAGTAGC TAGCGGTTCAGGACATCTCT 50.0 50.0 58.1 57.9 1051-1191 bp 1.0 90.3
psbM-trnD-GUC_p2 AGAATGAAGAGCGCAGTAGC TCTAGCGGTTCAGGACATCT 50.0 50.0 58.1 57.9 1053-1193 bp 1.0 90.3
psbM-trnD-GUC_p3 AATGAAGAGCGCAGTAGCAA TAGCGGTTCAGGACATCTCT 45.0 50.0 57.9 57.9 1049-1189 bp 1.0 90.0
psbM-trnD-GUC_p4 AATGAAGAGCGCAGTAGCAA TCTAGCGGTTCAGGACATCT 45.0 50.0 57.9 57.9 1051-1191 bp 1.0 90.0
psbM-trnD-GUC_p5 AGAATGAAGAGCGCAGTAGC GTCTAGCGGTTCAGGACATC 50.0 55.0 58.1 57.8 1054-1194 bp 1.0 89.8