Hotspot

atpF-atpH

LSC · 11877-12328 · 452 bp

Back to Arenaria

Pi 0.087

MarkerSeek score 83.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 59
pi: 0.087
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTT	50.0	45.0	58.0	56.8	473-538 bp	1.0	82.1
atpF-atpH_p2	CCAGTGACCCAAGAAAACGA	TTGGTTGTAGCATTAGCCCT	50.0	45.0	58.0	56.8	474-539 bp	1.0	82.1
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTTT	50.0	42.9	58.0	57.6	473-538 bp	1.0	80.1
atpF-atpH_p4	CCAGTGACCCAAGAAAACGA	TTGGTTGTAGCATTAGCCCTT	50.0	42.9	58.0	57.6	474-539 bp	1.0	80.1
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTTTT	50.0	40.9	58.0	58.2	473-538 bp	1.0	73.9