Hotspot

ycf1

SSC · 126858-132329 · 5472 bp

Pi 0.00716

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 72
pi: 0.00716
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCGGGGAGAACCAAAAGG	TCCATGCGTACTCAAAGACG	50.0	50.0	58.0	58.0	680 bp	1.0	82.3
ycf1_p2	TTTCGGGGAGAACCAAAAGG	CTGGAAGAACTGGAGGAGGA	50.0	55.0	58.0	58.1	896 bp	1.0	81.8
ycf1_p3	TTTCGGGGAGAACCAAAAGG	TCTGGAAGAACTGGAGGAGG	50.0	55.0	58.0	58.1	897 bp	1.0	81.8
ycf1_p4	TTTCGGGGAGAACCAAAAGG	TTCAAGAAAAGCCAAACGCG	50.0	45.0	58.0	58.2	840 bp	1.0	80.9
ycf1_p5	TTTCGGGGAGAACCAAAAGG	CCATGCGTACTCAAAGACGT	50.0	50.0	58.0	58.3	679 bp	1.0	80.1