Hotspot

rps15-ycf1

SSC · 126496-126857 · 362 bp

Back to Areca

Pi 0.0115
MarkerSeek score 48.50
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
362
variable_sites
8
pi
0.0115
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACGAGTTTTTGGTTCCCGA AATGGCAGCCGTTTCAGTAT 45.0 45.0 58.0 57.9 693 bp 1.0 80.0
rps15-ycf1_p2 ACGAGTTTTTGGTTCCCGAA AATGGCAGCCGTTTCAGTAT 45.0 45.0 58.0 57.9 692 bp 1.0 80.0
rps15-ycf1_p3 ACGAGTTTTTGGTTCCCGAA ATAATGGCAGCCGTTTCAGT 45.0 45.0 58.0 57.9 694 bp 1.0 80.0
rps15-ycf1_p4 GGCTCAGCTCCAGCATAATT AATGGCAGCCGTTTCAGTAT 50.0 45.0 58.0 57.9 1129 bp 1.0 79.9
rps15-ycf1_p5 GGCTCAGCTCCAGCATAATT ATAATGGCAGCCGTTTCAGT 50.0 45.0 58.0 57.9 1131 bp 1.0 79.9