Hotspot

rpl32-trnL-UAG

SSC · 116199-116987 · 789 bp

Back to Areca

Pi 0.00655
MarkerSeek score 60.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
789
variable_sites
10
pi
0.00655
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCCACCGGACATTCAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.4 58.0 881-884 bp 1.0 77.9
rpl32-trnL-UAG_p2 GGTTTCCACCGGACATTCAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.4 58.0 882-885 bp 1.0 77.9
rpl32-trnL-UAG_p3 GGTTTCCACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.4 57.9 891-894 bp 1.0 77.0
rpl32-trnL-UAG_p4 GGTTTCCACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.4 57.8 890-893 bp 1.0 76.4
rpl32-trnL-UAG_p5 GGTTTCCACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.4 57.8 889-892 bp 1.0 76.4