Hotspot

rpl22-rps19

LSC · 86347-86487 · 141 bp

Back to Areca

Pi 0.0402
MarkerSeek score 68.40
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
141
variable_sites
9
pi
0.0402
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCTTCTCTACCTGAACTCCAGT TACTTTCGCGAGACATGCAA 45.5 45.0 57.6 57.9 217-223 bp 1.0 64.3
rpl22-rps19_p2 TCTTCTCTACCTGAACTCCAGT TTACTTTCGCGAGACATGCA 45.5 45.0 57.6 57.9 218-224 bp 1.0 64.3
rpl22-rps19_p3 TCTTCTCTACCTGAACTCCAGT GAGAATTCGCGCCTACTCTT 45.5 50.0 57.6 57.8 236-242 bp 1.0 62.9
rpl22-rps19_p4 TCTTCTCTACCTGAACTCCAGT TTCGCGAGACATGCAAGAAA 45.5 45.0 57.6 58.5 213-219 bp 1.0 61.0
rpl22-rps19_p5 TCTTCTCTACCTGAACTCCAGT CAAATTGGGAGAATTCGCGC 45.5 50.0 57.6 58.5 244-250 bp 1.0 60.8