Hotspot

rps8-rpl14

LSC · 82849-83028 · 180 bp

Back to Areca

Pi 0.0148
MarkerSeek score 62.60
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
180
variable_sites
5
pi
0.0148
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGCTATTCGAACCGTTCCTT GAACTCGAGTTTTTGGTGCG 45.0 50.0 57.2 58.0 347-350 bp 1.0 75.0
rps8-rpl14_p2 GGCAATAGTGTCCCTACCCA GAACTCGAGTTTTTGGTGCG 55.0 50.0 58.8 58.0 287-290 bp 1.0 74.9
rps8-rpl14_p3 TGCTATTCGAACCGTTCCTT GCTCGGGAATTGAGACAGTT 45.0 50.0 57.2 57.9 324-327 bp 1.0 74.4
rps8-rpl14_p4 GGCAATAGTGTCCCTACCCA GCTCGGGAATTGAGACAGTT 55.0 50.0 58.8 57.9 264-267 bp 1.0 74.4
rps8-rpl14_p5 TGCTATTCGAACCGTTCCTT ACTCGAGTTTTTGGTGCGAT 45.0 45.0 57.2 58.1 345-348 bp 1.0 74.1