Hotspot

trnF-GAA-ndhJ

LSC · 49387-50109 · 723 bp

Back to Areca

Pi 0.00369
MarkerSeek score 62.10
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
723
variable_sites
5
pi
0.00369
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 833-851 bp 1.0 79.6
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 823-841 bp 1.0 79.0
trnF-GAA-ndhJ_p3 GGAAATGGTCGGGATAGCTC TGAAAGTTGGATAGGCTGGC 55.0 50.0 57.8 57.9 879-897 bp 1.0 78.6
trnF-GAA-ndhJ_p4 AATGGTCGGGATAGCTCAGT TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.2 57.9 876-894 bp 1.0 78.3
trnF-GAA-ndhJ_p5 ATGGTCGGGATAGCTCAGTT TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.2 57.9 875-893 bp 1.0 78.3