Hotspot

trnS-GCU-trnG-UCU

LSC · 8439-9791 · 1353 bp

Back to Areca

Pi 0.00683
MarkerSeek score 63.00
Primer available yes
Label trnS-GCU-trnG-UCU

Feature Metadata

label_name
trnS-GCU-trnG-UCU
feature_type
igs
region
LSC
length_bp
1353
variable_sites
17
pi
0.00683
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCU_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1318-1427 bp 1.0 56.4
trnS-GCU-trnG-UCU_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 1321-1430 bp 1.0 55.3
trnS-GCU-trnG-UCU_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 1320-1429 bp 1.0 55.3
trnS-GCU-trnG-UCU_p4 AGAAGAAGCGGAGAGAGAGG ACGAATCACACTTTTACCACT 55.0 38.1 58.2 55.5 1374-1483 bp 1.0 54.8
trnS-GCU-trnG-UCU_p5 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 1319-1428 bp 1.0 54.5