Hotspot

rpl22

LSC · 84110-84604 · 495 bp

Back to Ardisia

Pi 0.0238

MarkerSeek score 56.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 495
variable_sites: 32
pi: 0.0238
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTTGGCTGTGCAAACCAAAG	GAAAAGTCTGGGTAGAGCCG	45.0	55.0	58.0	58.0	1164-1170 bp	1.0	83.4
rpl22_p2	TTTGGCTGTGCAAACCAAAG	AGGTCACAAATTGGGCGAAT	45.0	45.0	58.0	58.1	683-688 bp	0.875	78.3
rpl22_p3	ACGCCACAGATAGTTCGAAC	GAAAAGTCTGGGTAGAGCCG	50.0	55.0	58.0	58.0	1701-1707 bp	0.875	77.4
rpl22_p4	ACGCCACAGATAGTTCGAAC	AGGTCACAAATTGGGCGAAT	50.0	45.0	58.0	58.1	1220-1225 bp	0.875	77.3
rpl22_p5	ACGCCACAGATAGTTCGAAC	TGGTAGGTCACAAATTGGGC	50.0	50.0	58.0	58.1	1224-1229 bp	0.875	77.2