Hotspot

rpl2-trnH-GUG

IRa · 154815-155081 · 267 bp

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Pi 0.0497
MarkerSeek score 65.50
Primer available yes
Label rpl2-trnH-GUG

Feature Metadata

label_name
rpl2-trnH-GUG
feature_type
igs
region
IRa
length_bp
267
variable_sites
26
pi
0.0497
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.38
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH-GUG_p1 AGTAAGCTGTGACGCGTTC ATTTGTGACCTACCATATGG 52.6 40.0 58.2 52.3 219 bp 0.375 16.5
rpl2-trnH-GUG_p2 GAGTAAGCTGTGACGCGTT ATTTGTGACCTACCATATGG 52.6 40.0 58.2 52.3 220 bp 0.375 16.5
rpl2-trnH-GUG_p3 AGTAAGCTGTGACGCGTTC AATTTGTGACCTACCATATGG 52.6 38.1 58.2 53.3 220 bp 0.375 16.5
rpl2-trnH-GUG_p4 AAAGGAGTAAGCTGTGACGC ATTTGTGACCTACCATATGG 50.0 40.0 58.2 52.3 224 bp 0.375 16.4
rpl2-trnH-GUG_p5 AAAGGAGTAAGCTGTGACGC AATTTGTGACCTACCATATGG 50.0 38.1 58.2 53.3 225 bp 0.375 16.4