Hotspot

rps15-ycf1

SSC · 124062-124482 · 421 bp

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Pi 0.0201

MarkerSeek score 61.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 421
variable_sites: 24
pi: 0.0201
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCCTGGGCTTTCCTCTTTTT	CGAACCTCTTCGTCTGTCTG	50.0	55.0	58.0	58.0	963-986 bp	0.75	76.4
rps15-ycf1_p2	TAAATAAGCGAGCAGGCGTT	CGAACCTCTTCGTCTGTCTG	45.0	55.0	58.0	58.0	1104-1127 bp	0.75	76.3
rps15-ycf1_p3	GCTTTCCCAGAATTTTCCGC	CGAACCTCTTCGTCTGTCTG	50.0	55.0	58.0	58.0	1081-1104 bp	0.625	71.2
rps15-ycf1_p4	GCGCTTTCCCAGAATTTTCC	CGAACCTCTTCGTCTGTCTG	50.0	55.0	58.0	58.0	1083-1106 bp	0.625	71.1
rps15-ycf1_p5	GGCGCTTTCCCAGAATTTTC	CGAACCTCTTCGTCTGTCTG	50.0	55.0	58.0	58.0	1084-1107 bp	0.625	71.1