Hotspot

ccsA-ndhD

SSC · 115934-116182 · 249 bp

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Pi 0.0394

MarkerSeek score 76.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 249
variable_sites: 21
pi: 0.0394
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTTGTGGCTTCTATTGGCT	TCAGTTGACAAGGTCGAAGT	45.0	45.0	57.4	56.7	422-457 bp	1.0	71.2
ccsA-ndhD_p2	TGTTGTGGCTTCTATTGGCT	TCAGTTGACAAGGTCGAAGTT	45.0	42.9	57.4	57.5	422-457 bp	1.0	69.1
ccsA-ndhD_p3	CTGTTGTGGCTTCTATTGGC	TCAGTTGACAAGGTCGAAGT	50.0	45.0	57.1	56.7	423-458 bp	1.0	68.5
ccsA-ndhD_p4	GCTGTTGTGGCTTCTATTGG	TCAGTTGACAAGGTCGAAGT	50.0	45.0	57.1	56.7	424-459 bp	1.0	68.5
ccsA-ndhD_p5	TGTTGTGGCTTCTATTGGCT	ATCAGTTGACAAGGTCGAAGT	45.0	42.9	57.4	57.2	423-458 bp	1.0	67.4