Hotspot

ycf1-ndhF

IRb · 110698-110710 · 13 bp

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Pi 0.201

MarkerSeek score 62.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRb
length_bp: 13
variable_sites: 6
pi: 0.201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TGAAACGAAAGAGAGCCGAG	GCGGACGCATCTCATCTTAT	50.0	50.0	57.9	57.9	177-204 bp	1.0	90.2
ycf1-ndhF_p2	GAAACGAAAGAGAGCCGAGT	GCGGACGCATCTCATCTTAT	50.0	50.0	57.9	57.9	176-203 bp	1.0	90.2
ycf1-ndhF_p3	TGAAACGAAAGAGAGCCGAG	CAAATATGTAGGGGGCGGAC	50.0	55.0	57.9	58.1	191-218 bp	1.0	89.4
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	GCGGACGCATCTCATCTTAT	50.0	50.0	58.0	57.9	361-388 bp	1.0	87.4
ycf1-ndhF_p5	GCAGAATACCGTCACCCATT	CAAATATGTAGGGGGCGGAC	50.0	55.0	58.0	58.1	375-402 bp	1.0	87.0