Hotspot

trnE-UUC-trnT-GGU

LSC · 30929-31709 · 781 bp

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Pi 0.00811

MarkerSeek score 62.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 18
pi: 0.00811
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAGAGAGATGTCCTGGACCG	GAACCGATGACTTACGCCTT	55.0	50.0	58.5	58.0	715-995 bp	1.0	81.4
trnE-UUC-trnT-GGU_p2	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	764-1044 bp	1.0	81.0
trnE-UUC-trnT-GGU_p3	AAGAGAGATGTCCTGGACCG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.5	58.1	694-974 bp	1.0	81.0
trnE-UUC-trnT-GGU_p4	AAGAGAGATGTCCTGGACCG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.5	58.1	691-971 bp	1.0	80.8
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.4	58.1	743-1023 bp	1.0	80.6