Hotspot

psbM-trnD-GUC

LSC · 29624-30539 · 916 bp

Back to Ardisia

Pi 0.0224
MarkerSeek score 69.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
916
variable_sites
69
pi
0.0224
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ACGAGAACGAACAATGCAGT TCAATTGGTTAGAGCACCGC 45.0 50.0 58.1 58.6 1029-1290 bp 1.0 80.1
psbM-trnD-GUC_p2 CACGAGAACGAACAATGCAG TCAATTGGTTAGAGCACCGC 50.0 50.0 57.8 58.6 1030-1291 bp 1.0 79.4
psbM-trnD-GUC_p3 GTAGGCACGAGAACGAACAA TCAATTGGTTAGAGCACCGC 50.0 50.0 58.2 58.6 1035-1296 bp 1.0 79.3
psbM-trnD-GUC_p4 CAGTAGGCACGAGAACGAAC TCAATTGGTTAGAGCACCGC 55.0 50.0 58.7 58.6 1037-1298 bp 1.0 75.8
psbM-trnD-GUC_p5 ACGAGAACGAACAATGCAGT AATTGGTTAGAGCACCGCC 45.0 52.6 58.1 58.1 1027-1288 bp 1.0 75.4