Hotspot

trnS-GCU-trnG-UCC

LSC · 8593-9330 · 738 bp

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Pi 0.0115

MarkerSeek score 60.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 23
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	AACCGAAAGACCCCTTAACT	55.0	45.0	58.6	56.4	613-835 bp	1.0	68.1
trnS-GCU-trnG-UCC_p2	GATTCGAACCCTCGGTACAA	AACCGAAAGACCCCTTAACT	50.0	45.0	57.3	56.4	664-886 bp	1.0	67.8
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	AAACCGAAAGACCCCTTAACT	55.0	42.9	58.6	57.1	614-836 bp	1.0	66.2
trnS-GCU-trnG-UCC_p4	GATTCGAACCCTCGGTACAA	AAACCGAAAGACCCCTTAACT	50.0	42.9	57.3	57.1	665-887 bp	1.0	65.9
trnS-GCU-trnG-UCC_p5	CGGAAAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	50.0	45.0	57.0	56.4	676-898 bp	1.0	65.3